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Diarrhea Profile (Dog) - PCR
Test number: 8168 |
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1 ) Canine Corona Virus (enteric) / CCV / CECoV / CCoV (Dog) PCR
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The Disease |
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An infection with canine (enteric) coronaviruses (CCoV, CCV, more recently also CECoV) is usually asymptomatic or may lead to mild, non-haemorrhagic diarrhoea.
In puppies, however, severe courses of the disease with haemorrhagic gastroenteritis are possible. Loss of intestinal villi, flattening of the epithelial cells of the small intestine and detachment of the goblet cells occur. The most noticeable signs are vomiting and watery to bloody diarrhoea, accompanied by severe dehydration. The virus is excreted via the faeces; the duration of excretion is usually less than two weeks.
CCV is also infectious to other canids as well as to cats and pigs, but the pathogenicity of these species is not yet known.
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Sample Requirements |
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FA .
faeces
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2 ) Parvo virus (Dog , Cat) - PCR
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Species
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Cat / Feline
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Dog / Canine
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Description |
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Realtime PCR
Parvoviruses
Parvovirus is a very small non-enveloped DNA virus with extreme environmental stability.
It can persist in the environment for up to a year and is also very temperature-resistant.
Animals become infected oronasally with parvoviruses. First, virus replication occurs in the mucous membranes, then followed by viraemia. The lymphatic system and organs become infected.
Dog
In dogs, parvovirus infection usually progresses as a cyclic systemic disease with a manifestationin the intestinal epithelium and the resulting clinical picture of anorexia, fever, vomiting and persistent bloody diarrhoea.
The disease is most severe in puppies. Different clinical forms of parvovirus infection can develop. The peracute form results in death within a few hours, usually without any serious signs. The acute form, however, is characterised by severe symptoms. High fever, severe bloody diarrhoea and vomiting occur. Due to the high affinity of the virus to tissues with high mitotic activity, severe leukopenia occurs simultaneously. If the leucocyte count falls below 2000 cells/µl, prognosis must be made carefully. Subclinically infected animals represent the pathogen reservoir as they shed the virus via the faeces.
Cat
Feline parvovirus infection – panleukopenia – is a highly contagious systemic disease of felids. The mortality rate among unvaccinated animals is over 80%.
Clinically, the disease is characterised by fever, diarrhoea, vomiting and dehydration. The blood count shows extreme leukopenia. A special case is the intrauterine infection. The mother cat is infected without showing any symptoms, but it leads to the abortion or death of the kittens. If kittens are born alive, there is often a cerebellar hypoplasia, which leads to ataxia and tremor, usually without any impairment of consciousness.
We also offer
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Sample Requirements |
Cat: FA , EB
Dog: FA, EB, GW .
in cats: faeces, EDTA blood.
In dogs: faeces, EDTA Blood 0.2 ml, tissue (e.g. intestine or heart).
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3 ) Dog Circovirus DogCV (Dog) PCR
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Sample Requirements |
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FA , EB .
faeces, EDTA blood (viraemia), tissue (e.g. liver, intestine, or kidney)
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4 ) Giardia -PCR
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Species
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Cat / Feline
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Dog / Canine
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Mammals
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Reptile
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Description |
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PCR
Giardia is a flagellate that can be found in the intestine of mammals, birds, reptiles, amphibians and humans. There are some well-differentiated species, such as G. intestinalis (lamblia, duodenalis). Giardia is ingested orally (food, water) or through smear infection as cysts, excystates in the intestine and attaches as trophozoites to the intestinal wall where it replicates. Damage to and detachment of the intestinal epithelium cause chronic intermittent catarrhal to mucous-bloody diarrhoea. The cysts that are excreted in the faeces remain infectious for many months in cold water and a humid environment.
Except for Giardia in birds and amphibians, Giardia is partially of zoonotic nature. Seven variants have been identified through genetic characterisation of which variants (assemblages) A and B mainly occur in humans, variants C and D are primarily detected in dogs and variant F can mostly be found in cats. Across species, however, isolates of different subtypes of A as well as those of B can be detected in different animal species, so that a transmission from humans to animals and from animals to humans cannot be excluded. In dogs and cats, Giardia is the predominant type of intestinal parasites. In our own examinations, Giardia infections were detected in 15% of cats; 3.5% of these animals contained the human-pathogenic assemblage A.
Note
- Giardia infections lead to a decrease in vitamin B 12.
- If treatment of Giardia fails in cats, Tritrichomonas foetus should also be considered.
- In case of a positive PCR result, testing for the presence of humanpathogenic assemblages A and B can subsequently be conducted.
Giardia in cats
- 15% of the animals are positive.
- 3.5% of the carriers are infectedwith human-pathogenic assemblage A.
We also offer
Giardia Antigen Ag) EIA test
Giardia can also be detected by
Endoparasites- flotation and SAFC
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5 ) Cryptosporidia - PCR
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Sample Requirements |
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FA , MSP .
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Price |
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to view the price please provide:
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To order:
- If you have any queries, please contact us on:
Tel: 0161 282 3066
email: info@laboklin.co.uk
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