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Francisella tularensis PCR

Test number: 8342

Species
Cat / Feline , Dog / Canine , Hare , Mice , Others , Rabbit .
The Disease
Francisella (F.) tularensis is a gram-negative, pleomorphic, non-motile, aerobically growing, rod-shaped bacterium that is very resistant especially in lower environmental temperatures.

This pathogen is the causative agent of the so-called tularaemia (rabbit fever), which is a zoonosis.

Four subspecies have been classified. Two subspecies are of particular clinical relevance: F. tularensis ssp. Tularensis and F. tularensis ssp. Holarctica, with F. tularensis ssp. Tularensis naturally occurring in North America only and being responsible for a more aggressive course of the disease. In contrast, F. tularensis ssp. Holarctica can be found in the entire northern hemisphere. It is predominantly hares, rabbits and rodents (mice) that are affected, but also numerous other animal species, including birds, are susceptible to this pathogen. In dogs, cats and sheep, sporadic cases of illness are known. Cats suffer from the disease more often than dogs, but overall, the disease is rarely contracted.

Symptoms of an acute disease are apathy, fever, tachypnoea and swelling of the lymph nodes; most animals die of septicaemia within 2 weeks. Furthermore, in a chronic course, emaciation and skin ulcerations occur; in dogs and cats in addition to splenomegaly, hepatomegaly, ulcers on the tongue and jaundice. Here, too, a lethal outcome is possible after 2 – 6 weeks.

The modes of transmission include blood-sucking insects like fleas, biting midges, lice, ticks etc., consumption of infected carcass/meat or contaminated water. The infectious dose is very low, only a few bacteria are sufficient (exception: infections through the gastrointestinal tract).

Sample Requirements
A, ln - Tissue (mainly spleen, liver, lung, kidney), lymph node (aspirate), swab without medium (pharynx, tonsil) .
Parameters
The PCR detects F. tularensis ssp. Holarctica
Price

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