LABOKLIN (UK)|Reptiles & Amphibians | Virus PCR tests| Skin Profile (Lizard)
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Skin Profile (Lizard)

Test number: 8562

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1 ) Adeno Virus
Species
Reptile .
Clinical Signs
Turtles/tortoises: Anorexia, oral lesions, diarrhea
Lizards: Enteritis, hepatitis
Snakes: : Enteritis, hepatitis
Description

Adenoviruses are mostly found in lizards and snakes, but have also been detected in turtles and tortoises. In these animals, infections have been associated with anorexia, oral lesions, and diarrhea. Adenoviruses can be detected in cloacal swabs in live animals and in liver and intestine of dead animals via PCR.

Our PCR detects adenoviruses of the genus Atadenovirus (mostly detected in different species of snakes and lizards, but also in tortoises) and also of the genus Testadenovirus (it is the proposed name for Adenoviruses found in different tortoise and turtle species).

Sample Requirements
swab (cloaca or pharynx), tracheal lavage, tissue (e.g. intestine or liver) .
Turnaround
1-2 weeks
2 ) Devriesea Agamarum (Lizard)
Species
Lizard .
Description

realtime PCR

The gram-positive bacterium Devriesea agamarum can cause dermatitis and cheilitis as well as septicaemia in lizards, predominantly in Uromastyx species, although other lizard species can also become infected. In the popular bearded dragons (Pogona vitticeps), asymptomatic courses are common, as Devriesea agamarum may be part of the oral microbiome in these animals. The potential transmission to susceptible animals should be taken into account when creating groups. Clinically, the disease often presents with yellow, scabby lesions in affected animals. Outbreaks with high mortality have been described, especially if septicaemia develops. The bacterium has been detected in both wild lizards as well as lizards in captivity.

Species: lizard, mainly Uromastyx as well as Pogona species

Sample Requirements
A, HT .

scabs, scales, skin scrapings, swab without medium (skin, oral cavity), tissue (skin biopsies, material of cheilitis, subcutaneous granulomas, organs if septicaemia is suspected), suspicious bacterial pure cultures of lizard skin

Turnaround
1-2 weeks
3 ) Rana Virus
Species
Amphibians , Fish , Reptile .
Description

Ranaviruses: Overview and Host Impact

Ranaviruses are enveloped, double-stranded DNA viruses belonging to the family Iridoviridae. They are globally distributed and possess a remarkably broad host range, infecting multiple animal classes including amphibians, reptiles, and fish. Transmission occurs via direct contact, environmental contamination, or cannibalism (ingestion of infected animals).

Amphibians

Ranaviruses are increasingly detected in amphibians and are associated with systemic disease and mass mortality. Two clinical forms are recognized:
  • Haemorrhagic form: marked by erythema (especially on the abdomen and upper legs), ulceration of the toes, and increased bleeding tendency.
  • Cutaneous form: may present with skin lesions.

    Some animals succumb rapidly without prior signs of illness, while others may act as inapparent carriers.

    Chelonians (Turtles and Tortoises)

    In chelonians, ranaviruses are linked to:
  • Stomatitis
  • Hepatitis
  • Rhinitis and pneumonia
  • Sudden death

    Detection in live animals is typically performed using oral swabs or blood samples. In deceased animals, liver, tongue, and skin are preferred tissues for virus identification.

    Lizards

    Ranavirus infection in lizards is associated with:
  • Skin lesions
  • Stomatitis
  • Granulomatous inflammation
  • Hepatitis
  • Mass mortality

    Diagnostic sampling includes oral swabs or skin in live animals, and liver, tongue, and skin in post-mortem cases.

    Snakes

    In snakes, ranaviruses have been observed to cause:
  • Granulomatous lesions in the oral cavity and liver
  • Stomatitis and hepatic changes

    Detection is carried out using oral swabs or liver samples.

    Fish

    Ranaviruses also affect fish, with presentations ranging from clinically inapparent infections to systemic disease with mass mortality.

  • Sample Requirements
    Snakes:Oral and cloacal swabs, liver
    Chelonian: Oral and cloacal swabs, blood, tongue, liver
    Lizards: Oral and cloacal swabs, skin, liver
    Fish: biopsy (gills), blood, tissue (e.g. Liver or kidney), swab (skin)
    Amphibians: biopsy (toe clips, tail clips), EB or drops of blood on filter paper, tissue (above all liver, kidney), perhaps swab without medium (skin) .
    Turnaround
    1-2 weeks
    4 ) Mycology (dermatophytes and yeasts)
    Species
    Reptile .
    Parameters
    Swab with medium, Tissue
    Turnaround
    1-2 weeks
    5 ) Nannizziopsis spp. PCR (yellow fungus disease)
    Species
    Reptile .
    Description

    realtime PCR

    Nannizziopsis spp.

    Keratinophilic fungi of the genus Nannizziopsis (N.) spp., order Onygenales, are known to cause a variety of skin alterations especially in lizards. The most common pathogen of this genus is Nannizziopsis guarroi.

    There are several other known species, like N. dermatitidis, N. chlamydospora, N. vriesii, N. crocodili und N. barbatae. Infections have been described worldwide and can be present in wild as well as captive animals. Central bearded dragons (Pogona vitticeps) are the most frequently affected lizard species, however, it can also be present in other reptiles. Infected animals commonly develop ulcerative or scabby dermatitis. Yellow discolourations are possible, for which the disease had been called “yellow fungus disease” in the past. Granulomas are common. Infections range from mild to severe, and can also affect internal organs. If left untreated, chronic infections develop progressively and can lead to the death of the animal. Transmission occurs via direct contact, but indirect transmission via the environment might also be possible.

    Nannizziopsis can cause skin disease in reptiles, which can progress to systemic and sometimes fatal disease.

    The skin lesions associated with this disease were previously referred to as 'yellow fungus disease'.

    Nannizziopsis spp. Infections have been reported in various lizard species, such as chameleons, geckos, dragon and iguana lizards, and crocodiles.

     
    Further reading
    Pathogenic skin fungi in Australian reptilesPDF file
    Sample Requirements
    Swab, deep skin scraping, scales, scabs .
    Turnaround
    1-3 weeks
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